Beauveria bassiana biogenic nanoparticles for the control of Noctuidae pests.

BACKGROUND: Biogenic metallic and oxide metal nanoparticles have potential as alternatives for several current problems in agriculture, such as the control of caterpillars which cause huge losses in the production of important crops. In the present study, capped and uncapped silver, iron oxide and titanium dioxide nanoparticles were synthesized from the filtrate of Beauveria bassiana and evaluated in regard to physico-chemical characteristics, capping composition, cytotoxicity, genotoxicity and biological activity on Helicoverpa armigera and Spodoptera frugiperda caterpillars. RESULTS: A difference in the physico-chemical parameters of the capped and uncapped nanoparticles was observed, with larger aggregation and lower stability of the uncapped. In regard to the study of the capping, the presence of functional groups of biomolecules as well as the activity of B. bassiana hydrolytic enzymes were observed. Cytotoxic effects on the tested cell lines were not observed and DNA damage levels increased with more intense effects of the uncapped nanoparticles. In regard to the biological activity against Noctuidae pests, the uncapped and capped iron oxide, and uncapped titanium dioxide nanoparticles occasioned higher mortality (76%, 60% and 51%, respectively) but no differences in LC50 were recorded. Moreover, sublethal effects were reported on Helicoverpa armigera whereas Spodoptera frugiperda showed low susceptibility to the nanoparticles. CONCLUSION: The results demonstrate that biogenic metallic and oxide metal nanoparticles might show promising effects for the control of caterpillars which cause damage on important agricultural crops. Further investigations are necessary to understand the mechanisms of action and optimize the biological activity of these new nanomaterials. © 2023 Society of Chemical Industry.

Zein based-nanoparticles loaded botanical pesticides in pest control: An enzyme stimuli-responsive approach aiming sustainable agriculture.

Nanoencapsulation of biopesticides is an important strategy to increase the efficiency of these compounds, reducing losses and adverse effects on non-target organisms. This study describes the preparation and characterisation of zein nanoparticles containing the botanical compounds limonene and carvacrol, responsive to proteolytic enzymes present in the insects guts. The spherical nanoparticles, prepared by the anti-solvent precipitation method, presented in the nanoparticle tracking analysis (NTA) a concentration of 4.7 × 1012 ± 1.3 × 1011 particles.mL-1 and an average size of 125 ± 2 nm. The formulations showed stability over time, in addition to not being phytotoxic to Phaseolus vulgaris plants. In vivo tests demonstrated that formulations of zein nanoparticles containing botanical compounds showed higher mortality to Spodoptera frugiperda larvae. In addition, the FTIC probe (fluorescein isothiocyanate) showed wide distribution in the larvae midgut, as well as being identified in the feces. The trypsin enzyme, as well as the enzymatic extract from insects midgut, was effective in the degradation of nanoparticles containing the mixture of botanical compounds, significantly reducing the concentration of nanoparticles and the changes in size distribution. The zein degradation was confirmed by the disappearance of the protein band in the electrophoresis gel, by the formation of the lower molecular weight fragments and also by the greater release of FTIC after enzymes incubation. In this context, the synthesis of responsive nanoparticles has great potential for application in pest management, increasing the selectivity and specificity of the system and contributing to a more sustainable agriculture.

Compatibility of Bt biopesticides and adjuvants for Spodoptera frugiperda control.

Spodoptera frugiperda is a pest of economic importance for several crops with resistance reports to Bt crops and pesticides. Eco-friendly Bt biopesticides may be an alternative to chemical insecticides due to their selectivity and specificity. However, the efficacy of Bt biopesticides may be influenced by the association with other chemicals, such as adjuvants. This study evaluated the compatibility and toxicity of Bt biopesticides mixed with adjuvants for the control of S. frugiperda. The treatments included the association of Dipel SC and Dipel PM with adjuvants. Compatibility tests were used to evaluate the Bt mixture. Bt suspensions obtained from mixtures of Bt and adjuvants at 106 and 3 × 108 spores/mL-1 were used to evaluate S. frugiperda mortality and distilled water was used as the control. The addition of the adjuvant LI increased growth and sporulation, indicating compatibility with Bt biopesticides. The other adjuvants were toxic to reducing Bt growth and sporulation. Only the mixture of Bt with LI and Bt alone was effective to S. frugiperda. The addition of adjuvants to Bt biopesticide affect the Bt sporulation, growth and mortality.

Association of zein nanoparticles with botanical compounds for effective pest control systems.

BACKGROUND: Botanical compounds from plant species are known to have pesticidal activity and have been used in integrated pest management programs. The varied spectrum of the pesticidal action of these compounds can also avoid selection of resistance in pest populations. In this study, mixtures of the botanical compounds geraniol, eugenol and cinnamaldehyde were encapsulated in zein nanoparticles to improve their stability and efficiency. Biological effects of the nano-scale formulations of the botanical compounds were evaluated against two agricultural pests: the two-spotted spider mite (Tetranychus urticae) and the soybean looper (Chrysodeixis includes). RESULTS: The formulations were stable over time (120 days) with a high encapsulation efficiency (>90%). Nanoencapsulation also provided protection against degradation of the compounds during storage and led to a decrease in toxicity to non-target organisms. The release of the compounds (especially eugenol and cinnamaldehyde) from the nanoparticles was directly influenced by temperature, and the main mechanism of release was through a diffusion-based process. Nanoencapsulated compounds also showed superior efficiency compared to the emulsified compounds in terms of repellency and insecticidal activity. CONCLUSION: The findings of this study indicate that the convergence of botanical compounds with nano-scale formulation has the potential to improve efficacy for their sustainable use in integrated pest management in agriculture. © 2019 Society of Chemical Industry.

Spodoptera albula susceptibility to Bacillus thuringiensis-based biopesticides.

Single concentration and virulence (mean lethal concentration) bioassays were performed to evaluate the susceptibility of S. albula second instar larvae to seven Bacillus thuringiensis-based biopesticides. Bioassays were conducted using three replicates and repeated three times at 25 °C, 70 ±â€¯10% RH, and a 12:12 (light/dark) photoperiod; mortality was recorded seven days after treatment. The results were subjected to a Tukey's test and Probit analysis. Agree, DiPel SC, and XenTari achieved mortality rates of up to 80%, with the first of these being the most virulent against S. albula. Different Dipel formulations showed different degrees of larvicidal activity.